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Registro Completo |
Biblioteca(s): |
Embrapa Agrossilvipastoril. |
Data corrente: |
03/02/2014 |
Data da última atualização: |
10/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. |
Afiliação: |
Maria Beatriz Calderan Rodrigues, USP-ESALQ; LEIA CECILIA DE LIMA FAVARO, CNPAE; Ana Paula de Souza Pallu, USP-ESALQ; ANDERSON FERREIRA, CPAMT; Fernanda de Souza Sebastianes, USP-ESALQ; Maria Juliana Calderan Rodrigues, USP-ESALQ; Marcel Bellato Spósito, USP-ESALQ; Welington Luiz DE Araújo, UMC; Aline Aparecida Pizzirani-Kleiner, USP-ESALQ. |
Título: |
Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013. |
ISSN: |
1878-6146 |
DOI: |
http://dx.doi.org/10.1016/j.funbio.2013.06.005 |
Idioma: |
Inglês |
Conteúdo: |
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction. MenosGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for ... Mostrar Tudo |
Palavras-Chave: |
Agrobacterium-Mediated; Plant-Pathogen Interaction. |
Thesaurus Nal: |
citrus black spot; genetic transformation; Guignardia citricarpa. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96268/1/cpamt-Ferreira-1878-6146-2013.pdf
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Marc: |
LEADER 02874naa a2200301 a 4500 001 1978312 005 2017-08-10 008 2013 bl uuuu u00u1 u #d 022 $a1878-6146 024 7 $ahttp://dx.doi.org/10.1016/j.funbio.2013.06.005$2DOI 100 1 $aRODRIGUES, M. B. C. 245 $aAgrobacterium-mediated transformation of Guignardia citricarpa$ban efficient tool to gene transfer and random mutagenesis.$h[electronic resource] 260 $c2013 520 $aGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction. 650 $acitrus black spot 650 $agenetic transformation 650 $aGuignardia citricarpa 653 $aAgrobacterium-Mediated 653 $aPlant-Pathogen Interaction 700 1 $aFAVARO, L. C. de L. 700 1 $aPALLU, A. P. de S. 700 1 $aFERREIRA, A. 700 1 $aSEBASTIANES, F. de S. 700 1 $aRODRIGUES, M. J. C. 700 1 $aSPÓSITO, M. B. 700 1 $aARAÚJO, W. L. de 700 1 $aPIZZIRANI-KLEINER, A. A. 773 $tFungal Biology, Oxford$gv. 117, n. 7/8, p. 556-568, jul./aug., 2013.
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Embrapa Agrossilvipastoril (CPAMT) |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Florestas; Embrapa Solos. |
Data corrente: |
19/07/2021 |
Data da última atualização: |
13/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
DEMETRIO, W. C.; CONRADO, A. C.; ACIOLI, A. N. S.; FERREIRA, A. C.; BARTZ, M. L. C.; JAMES, S. W.; SILVA, E. da; MAIA, L. S.; MARTINS, G. C.; MACEDO, R. S.; STANTON, D. W. G.; LAVELLE, P.; VELASQUEZ, E.; ZANGERLÉ, A.; BARBOSA, R.; TAPIA-CORAL, S. C.; MUNIZ, A. W.; SANTOS, A.; FERREIRA, T.; SEGALLA, R. F.; DECAËNS, T.; NADOLNY, H. S.; PEÑA-VENEGAS, C. P.; MAIA, C. M. B. F.; PASINI, A.; MOTA, A. F.; TAUBE JÚNIOR, P. S.; SILVA, T. A. C.; REBELLATO, L.; OLIVEIRA JUNIOR, R. C. de; NEVES, E. G.; LIMA, H. P.; FEITOSA, R. M.; TORRADO, P. V.; McKEY, D.; CLEMENT, C. R.; SHOCK, M. P.; TEIXEIRA, W. G.; MOTTA, A. C. V.; MELO, V. F.; DIECKOW, J.; GARRASTAZU, M. C.; CHUBATSU, L. S.; KILLE, P.; BROWN, G. G.; CUNHA, L. |
Afiliação: |
WILIAN C. DEMETRIO, Federal University of Paraná; ANA C. CONRADO, Federal University of Paraná; AGNO N. S. ACIOLI, UFAM; ALEXANDRE C. FERREIRA, Federal University of Paraná; MARIE L. C. BARTZ, University of Coimbra; SAMUEL W. JAMES, Maharishi International University; ELODIE DA SILVA, CNPF; LILIANNE S. MAIA, Federal University of Paraná; GILVAN COIMBRA MARTINS, CPAA; RODRIGO S. MACEDO, Instituto Nacional do Semiárido; DAVID W. G. STANTON, Swedish Museum of Natural History; PATRICK LAVELLE, Institut de Recherche pour le Développement; ELENA VELASQUEZ, Universidad Nacional de Colombia; ANNE ZANGERLÉ, Ministère de l’Agriculture, de la Viticulture et de la Protection des consommateurs; RAFAELLA BARBOSA, Centro Universitário do Norte; SANDRA C. TAPIA-CORAL, Servicio Nacional de Aprendizaje; ALEKSANDER WESTPHAL MUNIZ, CPAA; ALESSANDRA SANTOS, Federal University of Paraná; TALITA FERREIRA, Federal University of Paraná; RODRIGO F. SEGALLA, Federal University of Paraná; THIBAUD DECAËNS, CEFE, Univ Montpellier; HERLON S. NADOLNY, Federal University of Paraná; CLARA P. PEÑA-VENEGAS, Instituto Amazónico de Investigaciones Científicas SINCHI; CLÁUDIA M. B. F. MAIA, CNPF; AMARILDO PASINI, Universidade Estadual de Londrina; ANDRÉ F. MOTA, Federal University of Paraná; PAULO S. TAUBE JÚNIOR, Universidade Federal do Oeste do Pará; TELMA A. C. SILVA, INPA; LILIAN REBELLATO, Universidade Federal do Oeste do Pará; RAIMUNDO COSME DE OLIVEIRA JUNIOR, CPATU; EDUARDO G. NEVES, Museu de Arqueologia e Etnologia, Universidade de São Paulo; HELENA P. LIMA, Museu Paraense Emílio Goeldi; RODRIGO M. FEITOSA, Federal University of Paraná; PABLO VIDAL TORRADO, ESALQ; DOYLE MCKEY, CEFE, Univ Montpellier, CNRS; CHARLES R. CLEMENT, INPA; MYRTLE P. SHOCK, Universidade Federal do Oeste do Pará; WENCESLAU GERALDES TEIXEIRA, CNPS; ANTÔNIO C. V. MOTTA, Federal University of Paraná; VANDER F. MELO, Federal University of Paraná; JEFERSON DIECKOW, Federal University of Paraná; MARILICE CORDEIRO GARRASTAZU, CNPF; LEDA S. CHUBATSU, Federal University of Paraná; PETER KILLE, Cardiff University; GEORGE GARDNER BROWN, CNPF; LUÍS CUNHA, University of Coimbra. |
Título: |
A "dirty" footprint: macroinvertebrate diversity in Amazonian Anthropic soils. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Global Change Biology, v. 27, n. 19, p. 4575-4591, Oct. 2021. |
DOI: |
10.1111/gcb.15752 |
Idioma: |
Inglês |
Conteúdo: |
Amazonian rainforests, once thought to be pristine wilderness, are increasingly known to have been widely inhabited, modified, and managed prior to European arrival, by human populations with diverse cultural backgrounds. Amazonian Dark Earths (ADEs) are fertile soils found throughout the Amazon Basin, created by pre-Columbian societies with sedentary habits. Much is known about the chemistry of these soils, yet their zoology has been neglected. Hence, we characterized soil fertility, macroinvertebrate communities, and their activity at nine archeological sites in three Amazonian regions in ADEs and adjacent reference soils under native forest (young and old) and agricultural systems. |
Palavras-Chave: |
Amazonian Dark Earths; Ants; Archeological sites; Termites; Terra Preta de Índio. |
Thesagro: |
Biodiversidade; Biologia do Solo; Cupim; Fauna; Fertilidade do Solo; Formiga; Minhoca; Uso da Terra. |
Thesaurus NAL: |
Anthrosols; Earthworms; Formicidae; Land use change; Soil biology; Soil fauna; Soil fertility. |
Categoria do assunto: |
-- P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225924/1/gcb.15752.pdf
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Marc: |
LEADER 03074naa a2200913 a 4500 001 2133025 005 2021-09-13 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1111/gcb.15752$2DOI 100 1 $aDEMETRIO, W. C. 245 $aA "dirty" footprint$bmacroinvertebrate diversity in Amazonian Anthropic soils.$h[electronic resource] 260 $c2021 520 $aAmazonian rainforests, once thought to be pristine wilderness, are increasingly known to have been widely inhabited, modified, and managed prior to European arrival, by human populations with diverse cultural backgrounds. Amazonian Dark Earths (ADEs) are fertile soils found throughout the Amazon Basin, created by pre-Columbian societies with sedentary habits. Much is known about the chemistry of these soils, yet their zoology has been neglected. Hence, we characterized soil fertility, macroinvertebrate communities, and their activity at nine archeological sites in three Amazonian regions in ADEs and adjacent reference soils under native forest (young and old) and agricultural systems. 650 $aAnthrosols 650 $aEarthworms 650 $aFormicidae 650 $aLand use change 650 $aSoil biology 650 $aSoil fauna 650 $aSoil fertility 650 $aBiodiversidade 650 $aBiologia do Solo 650 $aCupim 650 $aFauna 650 $aFertilidade do Solo 650 $aFormiga 650 $aMinhoca 650 $aUso da Terra 653 $aAmazonian Dark Earths 653 $aAnts 653 $aArcheological sites 653 $aTermites 653 $aTerra Preta de Índio 700 1 $aCONRADO, A. C. 700 1 $aACIOLI, A. N. S. 700 1 $aFERREIRA, A. C. 700 1 $aBARTZ, M. L. C. 700 1 $aJAMES, S. W. 700 1 $aSILVA, E. da 700 1 $aMAIA, L. S. 700 1 $aMARTINS, G. C. 700 1 $aMACEDO, R. S. 700 1 $aSTANTON, D. W. G. 700 1 $aLAVELLE, P. 700 1 $aVELASQUEZ, E. 700 1 $aZANGERLÉ, A. 700 1 $aBARBOSA, R. 700 1 $aTAPIA-CORAL, S. C. 700 1 $aMUNIZ, A. W. 700 1 $aSANTOS, A. 700 1 $aFERREIRA, T. 700 1 $aSEGALLA, R. F. 700 1 $aDECAËNS, T. 700 1 $aNADOLNY, H. S. 700 1 $aPEÑA-VENEGAS, C. P. 700 1 $aMAIA, C. M. B. F. 700 1 $aPASINI, A. 700 1 $aMOTA, A. F. 700 1 $aTAUBE JÚNIOR, P. S. 700 1 $aSILVA, T. A. C. 700 1 $aREBELLATO, L. 700 1 $aOLIVEIRA JUNIOR, R. C. de 700 1 $aNEVES, E. G. 700 1 $aLIMA, H. P. 700 1 $aFEITOSA, R. M. 700 1 $aTORRADO, P. V. 700 1 $aMcKEY, D. 700 1 $aCLEMENT, C. R. 700 1 $aSHOCK, M. P. 700 1 $aTEIXEIRA, W. G. 700 1 $aMOTTA, A. C. V. 700 1 $aMELO, V. F. 700 1 $aDIECKOW, J. 700 1 $aGARRASTAZU, M. C. 700 1 $aCHUBATSU, L. S. 700 1 $aKILLE, P. 700 1 $aBROWN, G. G. 700 1 $aCUNHA, L. 773 $tGlobal Change Biology$gv. 27, n. 19, p. 4575-4591, Oct. 2021.
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